Cellulose is organic and is mainly used to separate hydrophilic compounds (soluble in aqueous solutions). This is true concerning bulk applications as well as Thin Layer Chromatography (TLC) related applications. The method for separation on cellulose is primarily of a partition nature. This means that the compounds to be separated are actually partitioned between the water coating the cellulose and the mostly aqueous mobile phase.
There are 2 types of normal cellulose powder that have been typically used for chromatography: native, fibrous cellulose and microcrystalline cellulose.
Characteristics of microcrystalline cellulose:
• Nominal particle size - 50um (microns)
• Moisture %: 3.0 - 5.0
• Bulk density: 0.26 - 0.31 g/cc
APPLICATIONS:
Carboxylic acids or carbohydrates, smaller chain alcohols, amino acids, urea and purine derivatives