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- HPTLC-HLF 150um 10x10cm w/Preadsorbent Zone (50 plates/box) P61077-2
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HPTLC-HLF 150um 10x10cm w/Preadsorbent Zone (50 plates/box) P61077-2
$426.00
SKU:
P61077-2
Weight:
7.20 LBS
Shipping:
Calculated at checkout
Product Description
| Adsorbent | Silica Gel, 10um average particle, 60Å pore |
| Backing | Glass |
| Size | 10x10 cm |
| Thickness | 150 um |
| Plates/box | 50 |
| UV254 (F) | Yes |
| Binder | Organic polymer |
| Compatibility | All typical chromatography solvents (80% max water) |
NOTE1: This box replaces P61077 (previously with only 25x plates per box)
The availability of both normal phase and Reversed Phase HPTLC UNIPLATES completes HPTLC as a sensible, efficient and cost effective alternative technique for quantitative analysis. These Reversed Phase adsorbents share the features and benefits of silica gel HPTLC plates: high efficiency adsorbents, 150 μm layer thickness, and smooth surface for densitometry.
In the High Performance format, Miles Scientific offers a variety of layers. A hydrocarbon impregnated layer, similar to the analytical RPS UNIPLATE, offers complete aqueous solvent compatibility. Three nonpolar bonded supports in the UNIBOND series are available: RP2, RP8, and RP18.
These fully silanized silica gels share complete compatibility with all organic solvents. However, due to the extreme hydrophobic nature of the RP18 adsorbent, aqueous wettability is limited to approximately 60% water in the mobile phase. Wettability is correspondingly higher with the slightly more polar shorter chain RP series plates. Selectivity can be varied for a given sample by choosing a UNIBOND Reversed Phase HPTLC plate of proper nonpolarity.
High Performance UNIPLATES provide high resolution separations in less than five minutes with migration distances as little as 5 to 7 cm.
These plates are prepared with high efficiency silica gel having a 10 micron average particle diameter and narrow particle size distribution. The adosrbent layers are only 150 microns thick, resulting in a more rapid solvent migration. The adsorbent capacity is more than adequate for small samples used in high performance TLC techniques. Typically, sample volumes for high performance TLC are less than one microliter. Samples spots are normally smaller than one millimeter in diameter.
Samples are spotted 1.0 to 1.5 cm from the bottom edge of the plate and 0.3 to 0.5 cm apart. Since smaller samples are applied, one 10 x 10 cm plate can separate as many samples as can be separated on a 20 x 20 cm plate using standard TLC techinques.
The smooth homogeneous surface of HPTLC UNIPLATES gives high signal-to-noise ratios for densitometric scanning, this increasing sensitivity and precision.
